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KMID : 0985420140360030122
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Laboratory Medicine and Quality Assurance
2014 Volume.36 No. 3 p.122 ~ p.139
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Annual Report on External Quality Assessment Scheme in Clinical Microbiology in Korea (2013)
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Ko Young-Jin
Kim Mi-Na
Kim Eui-Chong
Shin Jong-Hee
Lee Nam-Yong
Kim Sun-Joo
Jeong Seok-Hoon
Kim Jae-Seok
Kim Chang-Ki
Bae Hye-Gyung
Yoon Nam-Seob
Joo Sae-Ick
Hwang Yu-Yean
Kim Keon-Han
Jang In-Ho
Heo Jin
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Abstract
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Annual external quality assessment was performed three times for clinical microbiology division of The Korean Association of Quality Assurance for Clinical Laboratory. For each trial, three sets composed of different combinations of four bacteria and one yeast were distributed for culture, identification, and antimicrobial susceptibility tests. A total of 340 laboratories were enrolled and 330 (97.0%), 331(97.4%), and 331(97.4%) returned the results on trial I, II, and III, respectively. For bacterial identification, the correct identification of gram-negative bacilli, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus capitis, Streptococcus agalactiae, Listeria monocytogenes, and Candida species was greater than 95%. However, correct identification of Staphylococcus lugdunensis, Corynebacterium striatum, Vibrio vulnificus, Aeromonas hydrophila, Cryptococcus neoformans, and Malassezia pachydermatis was relatively less accurate, with values of 95.4%, 89.9%, 50.7%, 91.3%, 93.6%, and 93.9%, respectively. Surveillance cultures for vancomycin-resistant enterococci and methicillin-resistant S. aureus were correctly determined by 95.4% and 93.9% of the respondents, respectively. False carbapenem-resistance due to AmpC b-lactamase, disk diffusion testing for vancomycin in Staphylococcus species, oxacillin and penicillin susceptibility testing in S. lugdunensis and false imipenem-resistance in Proteus species were common sources of inaccurate results. The accuracy of species identification for Corynebacterium species and Vibrio species requires improvement. Consistent problems occurred with antimicrobial susceptibility testing of vancomycin for Staphylococcus species using the disk diffusion method.
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KEYWORD
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External quality assessment, Clinical laboratory, Bacterial culture, Antimicrobial susceptibility test
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